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Image Search Results


Journal: Stem cell research

Article Title: Generation of three induced pluripotent stem cells lines from patients with esophageal atresia/tracheoesophageal fistula type C

doi: 10.1016/j.scr.2022.102711

Figure Lengend Snippet:

Article Snippet: Antibodies used for Immunohistochemistry/Immunofluorescence Antibody Dilution Company Cat # RRID Pluripotency marker Rabbit anti-human SOX2 1 is to 200 Life Technologies Cat# 48–1400 AB_2533841 Pluripotency marker Mouse anti-human OCT4 1 is to 100 Biolegend Cat #653702 AB_2561766 Pluripotency marker Mouse anti-human SSEA4 1 is to 200 ThermoFisher Cat# 41–4000 AB_2533506 Pluripotency marker Mouse anti-human TRA-1–60 1 is to 200 BioLegend Cat# 330602 AB_1186144 Secondary antibody anti-rabbit Alexa 488 1 is to 1000 ThermoFisher Cat#A11070 AB_142134 Secondary antibody Anti-mouse Alexa 488 1 is to 1000 ThermoFisher Cat# A28175 AB_2536161 Secondary antibody Anti-mouse Alexa 594 1 is to 1000 ThermoFisher Cat# A11020 AB_141974 Nuclear counterstain DAPI 2ug/mL Thermo Fisher Cat# D1306 AB_2629482 Primers Target Size of band Thermo Fisher TaqMan assay ID Sendai virus and transgenes Sendai-cMyc 89bp Mr04269876_mr Sendai virus and transgenes Sendai-OCT3/4 82bp Mr04269878_mr Sendai virus and transgenes Sendai-KLF4 74bp Mr04269879_mr Sendai virus and transgenes Sendai 59bp Mr04269880_mr Sendai virus and transgenes Sendai-SOX2 62bp Mr04269881_mr Pluripotency marker SOX2 86bp Hs04234836_s1 Pluripotency marker NANOG 109bp Hs02387400_g1 Pluripotency marker OTC4 77bp Hs00999632_g1 Housekeeping gene GAPDH 157bp Hs02786624_g1 Open in a separate window Reagent details.

Techniques: Virus, Expressing, Modification

Characterization and validation

Journal: Stem cell research

Article Title: Generation of three induced pluripotent stem cells lines from patients with esophageal atresia/tracheoesophageal fistula type C

doi: 10.1016/j.scr.2022.102711

Figure Lengend Snippet: Characterization and validation

Article Snippet: Antibodies used for Immunohistochemistry/Immunofluorescence Antibody Dilution Company Cat # RRID Pluripotency marker Rabbit anti-human SOX2 1 is to 200 Life Technologies Cat# 48–1400 AB_2533841 Pluripotency marker Mouse anti-human OCT4 1 is to 100 Biolegend Cat #653702 AB_2561766 Pluripotency marker Mouse anti-human SSEA4 1 is to 200 ThermoFisher Cat# 41–4000 AB_2533506 Pluripotency marker Mouse anti-human TRA-1–60 1 is to 200 BioLegend Cat# 330602 AB_1186144 Secondary antibody anti-rabbit Alexa 488 1 is to 1000 ThermoFisher Cat#A11070 AB_142134 Secondary antibody Anti-mouse Alexa 488 1 is to 1000 ThermoFisher Cat# A28175 AB_2536161 Secondary antibody Anti-mouse Alexa 594 1 is to 1000 ThermoFisher Cat# A11020 AB_141974 Nuclear counterstain DAPI 2ug/mL Thermo Fisher Cat# D1306 AB_2629482 Primers Target Size of band Thermo Fisher TaqMan assay ID Sendai virus and transgenes Sendai-cMyc 89bp Mr04269876_mr Sendai virus and transgenes Sendai-OCT3/4 82bp Mr04269878_mr Sendai virus and transgenes Sendai-KLF4 74bp Mr04269879_mr Sendai virus and transgenes Sendai 59bp Mr04269880_mr Sendai virus and transgenes Sendai-SOX2 62bp Mr04269881_mr Pluripotency marker SOX2 86bp Hs04234836_s1 Pluripotency marker NANOG 109bp Hs02387400_g1 Pluripotency marker OTC4 77bp Hs00999632_g1 Housekeeping gene GAPDH 157bp Hs02786624_g1 Open in a separate window Reagent details.

Techniques: Microscopy, Immunofluorescence, Staining, Expressing, Mutagenesis, Sequencing, Variant Assay